ABSTRACT
A crucial role in eliciting anti-tumor immunity of costimulatory molecule CD80(B7-1) has been demonstrated by animal experimental studies.In this paper, CD80 expression in human tumor cell lines and EBV-transformed B cell was detected using RT-PCR and FACS methods. The results showed that CD80 expression of Raji and EBV-transformed B cell was positive but that of 3AO,MCF-7,MDA-453,MKN-45, Hela was negative. We have constructed retroviral vector CD80-pLN,transfected package cell PA317, screened high titer rctrovirus supernatant then infected human tumor cell lines and gained CD80 positive tumor cell clones. With pre-and post-CD80-transfected human breast carcinoma cell line MDA-453,the upregulated expression of ICAM-I, HLA class I molecule was observed, but the expression of HLA class II molecule wasn't changed.
ABSTRACT
This experiment takes the apute experimental serum sickness (AESS) in rabbits as an animal model to investigate the mechanism of the regulatory effects of Dexamethasone (DX) . The results show. DX doesn't inhibit the production of anti-BSA antibody but induces the tendency to increase the level of antibody in rabbits with DX administration on early days (EL, EH); immune complexes(BSA - anti-BSA IGg, CIC) in the rabbits of EL and EH are significantly increased comparing with the later days (LE, LH) and positive controls. Non-specific esterase stainings and the pathological changes of kidney sections are markedly lessened though the amount of anti - BSA antibody, circulating immune complex (CIC) and superoxides dismutase activity in the rabbits of LE and L H are the same as the positive control. The outcome of protecting kidneys of AESS rabbits from injury by DX administration . probadly results from the effects of that DX inhibits infiltration of monocytes into the glomeruli. The role of oxygen radicals in AESS needs further investigation.